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  • CONFOCAL MICROSCOPY
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    CONFOCAL MICROSCOPY
    ABELSON, JOHN N. / SIMON, MELVIN I.
    This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen B...

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  • ENZYME KINETICS AND MECHANISMS, PART E, ENERGETICS OF ENZYME CATALYSIS
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    ENZYME KINETICS AND MECHANISMS, PART E, ENERGETICS OF ENZYME CATALYSIS
    ABELSON, JOHN N. / SIMON, MELVIN I.
    This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen B...

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  • AMYLOID, PRIONS, AND OTHER PROTEIN AGGREGATES
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    AMYLOID, PRIONS, AND OTHER PROTEIN AGGREGATES
    ABELSON, JOHN N. / SIMON, MELVIN I.
    This volume includes a core of methodologies to attack the unique experimental problems presented by protein misassembly. Emphasis is on human biology applications, the area in which there is the most interest, in which most of the work has already been done, and in which there is the best evidence for the structural sophisitication of the protein aggregates.The critically accl...

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  • BIOFILMS
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    BIOFILMS
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Volume 310 of Methods in Enzymology is the first volume devoted solely to biofilm research methods. It provides a contemporary source book for virtually any kind of experimental approach involving biofilms. It includes bioengineering, molecular, genetic, microscopic, chemical, continuous culture, and physical methods. This volume will serve as a starting point for future develo...

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  • SPHINGOLIPID METABOLISM AND CELL SIGNALING, PART B
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    SPHINGOLIPID METABOLISM AND CELL SIGNALING, PART B
    ABELSON, JOHN N. / SIMON, MELVIN I.
    This volume contains information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets. Its companion Volume 311 presents methods used in studying enzymes of sphingolipid biosynthesis and turnover, including inhibitors of some of these enzymes, genetic approaches, and organic and enzymatic syntheses of sph...

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  • ANTISENSE TECHNOLOGY, PART B: APPLICATIONS: APPLICATIONS
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    ANTISENSE TECHNOLOGY, PART B: APPLICATIONS: APPLICATIONS
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods which inhibit gene expression permit studies probing the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmaco...

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  • VERTEBRATE PHOTOTRANSDUCTION AND THE VISUAL CYCLE, PART A
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    VERTEBRATE PHOTOTRANSDUCTION AND THE VISUAL CYCLE, PART A
    ABELSON, JOHN N.
    This volume, and its companion Volume 316, include newly developed methods to study vertebrate phototransduction and the visual cycle. Major topics covered include expression, isolation, and characterization of opsins; proteins that interact with rhodopsin; transducin and regulators of G-protein signaling; photoreceptor protein phosphatases, phosphodiesterase, and guanylylcycla...

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  • REGULATORS AND EFFECTORS OF SMALL GTPASES, PART E: GTPASES INVOLVED IN VESICULAR TRAFFIC
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    REGULATORS AND EFFECTORS OF SMALL GTPASES, PART E: GTPASES INVOLVED IN VESICULAR TRAFFIC
    JOHN N. ABELSON
    Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport.This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325) cover all biochemical and biologi...

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  • HYPERTHEROMPHILIC ENZYMES, PART A
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    HYPERTHEROMPHILIC ENZYMES, PART A
    ABELSON, JOHN N.
    The critically acclaimed laboratory standard for more than forty years, Methods in Enzymologyis one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much ma...

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  • REGULATORS AND EFFECTORS OF SMALL GTPASES, PART F: RAS FAMILY I: RAS FAMILY I
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    REGULATORS AND EFFECTORS OF SMALL GTPASES, PART F: RAS FAMILY I: RAS FAMILY I
    ABELSON, JOHN N. / SIMON, MELVIN I.
    The critically acclaimed laboratory standard for more than 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much mate...

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  • GHRELIN
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    GHRELIN
    ABELSON, JOHN N.
    This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The volume covers ghrelin, and has chapters on such topics as orphan gpcrs and methods for identifying their ligands, ghrelin o-acyltransferase assays and inhibition, and thermogenic characterization of ghrelin receptor null mice....

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  • BIOLUMINESCENCE AND CHEMILUMINESCENCE, PART C
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    BIOLUMINESCENCE AND CHEMILUMINESCENCE, PART C
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Since the publication of Bioluminescence and Chemiluminescence, Part B, genes have been cloned that encode luciferases from an array of bioluminescent organisms, novel applications of these genes have been developed, and much has been learned of the fundamental chemistry, biochemistry, structural biology, and biophysics of these intriguing enzymes. New strategies for applicatio...

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  • SPHINGOLIPID METABOLISM AND CELL SIGNALING, PART A
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    SPHINGOLIPID METABOLISM AND CELL SIGNALING, PART A
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Sphingolipids are found in all eukaryotic and in some prokaryotic organisms and provide structure for cell membranes, lipoproteins, and other biological materials as well as participate in the regulation of cell growth, differentiation, and diverse cell functions, including cell-cell communication, cell-substratum interactions, and intracellular signal transduction. This volume...

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  • ANTISENSE TECHNOLOGY, PART A, GENERAL METHODS, METHODS OF DELIVERY, AND RNA STUDIES
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    ANTISENSE TECHNOLOGY, PART A, GENERAL METHODS, METHODS OF DELIVERY, AND RNA STUDIES
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods that inhibit gene expression permit studies which probe the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharm...

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  • VERTEBRAE PHOTOTRANSDUCTION AND THE VISUAL CYCLE, PART B
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    VERTEBRAE PHOTOTRANSDUCTION AND THE VISUAL CYCLE, PART B
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Major topics covered include photoreceptor proteins, phototransduction calcium-binding proteins and calcium measurement in photoreceptor cells, enzymes of the visual cycle, posttranslational and chemical modifications, analysis of animal models of retinal diseases. Inherited retinal disease; from the defective gene to its function and repair. This volume and its companion Volum...

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  • RNA - LIGAND INTERACTIONS, PART A: STRUCTURAL BIOLOGY METHODS: STRUCTURAL BIOLOGY METHODS
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    RNA - LIGAND INTERACTIONS, PART A: STRUCTURAL BIOLOGY METHODS: STRUCTURAL BIOLOGY METHODS
    ABELSON, JOHN N. / SIMON, MELVIN I.
    RNA-Ligand Interactions, Part A focuses on structural biology methods. Major topics covered include semisynthetic methodologies (RNA synthetic methods and derivatization of RNA); RNA structure determination (X-ray crystallography, NMR, EM); techniques for monitoring RNA conformation and dynamics (solution methods and electrophoretic and spectroscopic methods); and modeling tert...

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  • SINGLET OXYGEN, UV-A AND OZONE
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    SINGLET OXYGEN, UV-A AND OZONE
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Recent advances in understanding the biological role of singlet oxygen in the pathways of cellular responses to ultraviolet-A radiation: its key position in photodynamical effects, and its generation by photochemical (dark) reactions, e.g. by cells of the immune system such as eosinophils and macrophages, are the focus of this volume. The new methods and techniques responsible ...

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  • APOPTOSIS
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    APOPTOSIS
    JOHN N. ABELSON
    Volume 322 of Methods in Enzymology is dedicated to apoptosis. Major topics covered include measuring apoptosis and apoptosis-induced endonucleases, measuring apoptosis in lower organisms, proteases involved in apoptosis and their inhibitors, cell free systems for monitoring steps in apoptosis pathways, mitochondria and apoptosis, bCl-2 family proteins, and studying receptors a...

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  • ENERGETICS OF BIOLOGICAL MACROMOLECULES, PART C
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    ENERGETICS OF BIOLOGICAL MACROMOLECULES, PART C
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Volume 323 of Methods in Enzymology is dedicated to the energetics of biological macromolecules. Understanding the molecular mechanisms underlying a biological process requires detailed knowledge of the structural relationships within the system and an equally detailed understanding of the energetic driving forces that control the structural interactions. This volume presents m...

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  • BRANCHED-CHAIN AMINO ACIDS, PART B
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    BRANCHED-CHAIN AMINO ACIDS, PART B
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Volume 324 of Methods in Enzymology supplements Volume 166. It includes genetic information (cloning, gene expression) and information on human genetic diseases not available when Volume 166 was published.General Description of the Series:The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publication...

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  • REGULATORS AND EFFECTORS OF SMALL GTPASES, PART D: RHO FAMILY: RHO FAMILY
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    REGULATORS AND EFFECTORS OF SMALL GTPASES, PART D: RHO FAMILY: RHO FAMILY
    ABELSON, JOHN N. / SIMON, MELVIN I.
    This volume and its companions (Volumes 255, 256, 257, and the forthcoming 329) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications ...

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