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  • CONFOCAL MICROSCOPY
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    CONFOCAL MICROSCOPY
    ABELSON, JOHN N. / SIMON, MELVIN I.
    This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen B...

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  • ENZYME KINETICS AND MECHANISMS, PART E, ENERGETICS OF ENZYME CATALYSIS
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    ENZYME KINETICS AND MECHANISMS, PART E, ENERGETICS OF ENZYME CATALYSIS
    ABELSON, JOHN N. / SIMON, MELVIN I.
    This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen B...

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  • AMYLOID, PRIONS, AND OTHER PROTEIN AGGREGATES
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    AMYLOID, PRIONS, AND OTHER PROTEIN AGGREGATES
    ABELSON, JOHN N. / SIMON, MELVIN I.
    This volume includes a core of methodologies to attack the unique experimental problems presented by protein misassembly. Emphasis is on human biology applications, the area in which there is the most interest, in which most of the work has already been done, and in which there is the best evidence for the structural sophisitication of the protein aggregates.The critically accl...

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  • BIOFILMS
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    BIOFILMS
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Volume 310 of Methods in Enzymology is the first volume devoted solely to biofilm research methods. It provides a contemporary source book for virtually any kind of experimental approach involving biofilms. It includes bioengineering, molecular, genetic, microscopic, chemical, continuous culture, and physical methods. This volume will serve as a starting point for future develo...

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  • SPHINGOLIPID METABOLISM AND CELL SIGNALING, PART B
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    SPHINGOLIPID METABOLISM AND CELL SIGNALING, PART B
    ABELSON, JOHN N. / SIMON, MELVIN I.
    This volume contains information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets. Its companion Volume 311 presents methods used in studying enzymes of sphingolipid biosynthesis and turnover, including inhibitors of some of these enzymes, genetic approaches, and organic and enzymatic syntheses of sph...

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  • ANTISENSE TECHNOLOGY, PART B: APPLICATIONS: APPLICATIONS
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    ANTISENSE TECHNOLOGY, PART B: APPLICATIONS: APPLICATIONS
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods which inhibit gene expression permit studies probing the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmaco...

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  • REGULATORS AND EFFECTORS OF SMALL GTPASES, PART F: RAS FAMILY I: RAS FAMILY I
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    REGULATORS AND EFFECTORS OF SMALL GTPASES, PART F: RAS FAMILY I: RAS FAMILY I
    ABELSON, JOHN N. / SIMON, MELVIN I.
    The critically acclaimed laboratory standard for more than 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much mate...

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  • TWO-COMPONENT SIGNALING SYSTEMS, PART A
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    TWO-COMPONENT SIGNALING SYSTEMS, PART A
    SIMON, MELVIN I.
    Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." Sections include: Computational Analyses of Sequences and Sequence Alignments Biochemical and Genetic Assays of Individual Components of Signaling System...

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  • TWO-COMPONENT SIGNALING SYSTEMS, PART B
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    TWO-COMPONENT SIGNALING SYSTEMS, PART B
    SIMON, MELVIN I.
    Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." Sections include:Structural ApproachesReconstitution of Heterogeneous SystemsIntracellular Methods and AssaysGenome-Wide Analyses of Two-Component System...

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  • CONSTITUTIVE ACTIVITY IN RECEPTORS AND OTHER PROTEINS, PART B
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    CONSTITUTIVE ACTIVITY IN RECEPTORS AND OTHER PROTEINS, PART B
    SIMON, MELVIN I.
    This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume.A. Expert authors wh...

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  • CONSTITUTIVE ACTIVITY IN RECEPTORS AND OTHER PROTEINS, PART A
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    CONSTITUTIVE ACTIVITY IN RECEPTORS AND OTHER PROTEINS, PART A
    MELVIN I. SIMON
    This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume.A. Expert authors wh...

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  • BIOTHERMODYNAMICS, PART C
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    BIOTHERMODYNAMICS, PART C
    SIMON, MELVIN I.
    In the past several years, there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts of data on their systems. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. The use of thermodynamics in biological resear...

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  • COMPUTER METHODS, PART C
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    COMPUTER METHODS, PART C
    MELVIN I. SIMON
    The combination of faster, more advanced computers and more quantitatively oriented biomedical researchers has recently yielded new and more precise methods for the analysis of biomedical data. These better analyses have enhanced the conclusions that can be drawn from biomedical data, and they have changed the way that experiments are designed and performed. This volume, along ...

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  • TWO-COMPONENT SIGNALING SYSTEMS, PART C
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    TWO-COMPONENT SIGNALING SYSTEMS, PART C
    MELVIN I. SIMON
    Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." This volume, the third in a three-volume treatment edited by the same group of editors, includes a wide range of methods, including those dealing with th...

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  • BIOLUMINESCENCE AND CHEMILUMINESCENCE, PART C
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    BIOLUMINESCENCE AND CHEMILUMINESCENCE, PART C
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Since the publication of Bioluminescence and Chemiluminescence, Part B, genes have been cloned that encode luciferases from an array of bioluminescent organisms, novel applications of these genes have been developed, and much has been learned of the fundamental chemistry, biochemistry, structural biology, and biophysics of these intriguing enzymes. New strategies for applicatio...

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  • SPHINGOLIPID METABOLISM AND CELL SIGNALING, PART A
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    SPHINGOLIPID METABOLISM AND CELL SIGNALING, PART A
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Sphingolipids are found in all eukaryotic and in some prokaryotic organisms and provide structure for cell membranes, lipoproteins, and other biological materials as well as participate in the regulation of cell growth, differentiation, and diverse cell functions, including cell-cell communication, cell-substratum interactions, and intracellular signal transduction. This volume...

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  • ANTISENSE TECHNOLOGY, PART A, GENERAL METHODS, METHODS OF DELIVERY, AND RNA STUDIES
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    ANTISENSE TECHNOLOGY, PART A, GENERAL METHODS, METHODS OF DELIVERY, AND RNA STUDIES
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods that inhibit gene expression permit studies which probe the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharm...

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  • VERTEBRAE PHOTOTRANSDUCTION AND THE VISUAL CYCLE, PART B
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    VERTEBRAE PHOTOTRANSDUCTION AND THE VISUAL CYCLE, PART B
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Major topics covered include photoreceptor proteins, phototransduction calcium-binding proteins and calcium measurement in photoreceptor cells, enzymes of the visual cycle, posttranslational and chemical modifications, analysis of animal models of retinal diseases. Inherited retinal disease; from the defective gene to its function and repair. This volume and its companion Volum...

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  • RNA - LIGAND INTERACTIONS, PART A: STRUCTURAL BIOLOGY METHODS: STRUCTURAL BIOLOGY METHODS
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    RNA - LIGAND INTERACTIONS, PART A: STRUCTURAL BIOLOGY METHODS: STRUCTURAL BIOLOGY METHODS
    ABELSON, JOHN N. / SIMON, MELVIN I.
    RNA-Ligand Interactions, Part A focuses on structural biology methods. Major topics covered include semisynthetic methodologies (RNA synthetic methods and derivatization of RNA); RNA structure determination (X-ray crystallography, NMR, EM); techniques for monitoring RNA conformation and dynamics (solution methods and electrophoretic and spectroscopic methods); and modeling tert...

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  • RNA-LIGAND INTERACTIONS, PART B: MOLECULAR BIOLOGY METHODS: MOLECULAR BIOLOGY METHODS
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    RNA-LIGAND INTERACTIONS, PART B: MOLECULAR BIOLOGY METHODS: MOLECULAR BIOLOGY METHODS
    SIMON, MELVIN I.
    RNA-Ligand Interactions, Part B focuses on molecular biology methods. Major topics covered include: solution probe methods, tethered-probe methodologies, in vitro affinity selection methodologies, genetic methodologies for detecting RNA-protein interactions, protein engineering methodologies useful for RNA-protein interaction studies, and cell biology methods.RNA-Ligand Interac...

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  • SINGLET OXYGEN, UV-A AND OZONE
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    SINGLET OXYGEN, UV-A AND OZONE
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Recent advances in understanding the biological role of singlet oxygen in the pathways of cellular responses to ultraviolet-A radiation: its key position in photodynamical effects, and its generation by photochemical (dark) reactions, e.g. by cells of the immune system such as eosinophils and macrophages, are the focus of this volume. The new methods and techniques responsible ...

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  • ENERGETICS OF BIOLOGICAL MACROMOLECULES, PART C
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    ENERGETICS OF BIOLOGICAL MACROMOLECULES, PART C
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Volume 323 of Methods in Enzymology is dedicated to the energetics of biological macromolecules. Understanding the molecular mechanisms underlying a biological process requires detailed knowledge of the structural relationships within the system and an equally detailed understanding of the energetic driving forces that control the structural interactions. This volume presents m...

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  • BRANCHED-CHAIN AMINO ACIDS, PART B
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    BRANCHED-CHAIN AMINO ACIDS, PART B
    ABELSON, JOHN N. / SIMON, MELVIN I.
    Volume 324 of Methods in Enzymology supplements Volume 166. It includes genetic information (cloning, gene expression) and information on human genetic diseases not available when Volume 166 was published.General Description of the Series:The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publication...

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  • REGULATORS AND EFFECTORS OF SMALL GTPASES, PART D: RHO FAMILY: RHO FAMILY
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    REGULATORS AND EFFECTORS OF SMALL GTPASES, PART D: RHO FAMILY: RHO FAMILY
    ABELSON, JOHN N. / SIMON, MELVIN I.
    This volume and its companions (Volumes 255, 256, 257, and the forthcoming 329) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications ...

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  • APPLICATIONS OF CHIMERIC GENES AND HYBRID PROTEINS, PART A: GENE EXPRESSION AND PROTEIN PURIFICATION: GENE EXPRESSION AND PROTEIN PURIFICATION
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    APPLICATIONS OF CHIMERIC GENES AND HYBRID PROTEINS, PART A: GENE EXPRESSION AND PROTEIN PURIFICATION: GENE EXPRESSION AND PROTEIN PURIFICATION
    SIMON, MELVIN I.
    The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much m...

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  • APPLICATIONS OF CHIMERIC GENES AND HYBRID PROTEINS, PART B: CELL BIOLOGY AND PHYSIOLOGY: CELL BIOLOGY AND PHYSIOLOGY
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    APPLICATIONS OF CHIMERIC GENES AND HYBRID PROTEINS, PART B: CELL BIOLOGY AND PHYSIOLOGY: CELL BIOLOGY AND PHYSIOLOGY
    SIMON, MELVIN I.
    The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much m...

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  • APPLICATIONS OF CHIMERIC GENES AND HYBRID PROTEINS, PART C: PROTEIN-PROTEIN INTERACTIONS AND GENOMICS: PROTEIN-PROTEIN INTERACTIONS AND GENOMICS
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    APPLICATIONS OF CHIMERIC GENES AND HYBRID PROTEINS, PART C: PROTEIN-PROTEIN INTERACTIONS AND GENOMICS: PROTEIN-PROTEIN INTERACTIONS AND GENOMICS
    SIMON, MELVIN I.
    The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much m...

    $2,792.00

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