Genetic Engineering 1 is the first of a series containing reviews of particular topics using genetic recombinant DNA techniques. This three-chapter volume describes the construction of libraries of expressed gene sequences, the use of gene-specific probes in antenatal diagnosis, and the expression of isolated genes in cellular and cell-free systems. Chapter 1 presents particular series of steps for the preparation and screening of cDNA clone bank. Chapter 2 discusses the advances in DNA analysis techniques that have profound effects upon the understanding of some genetic diseases and on the ability to reduce the incidence of these diseases by antenatal diagnosis and therapeutic abortion. Chapter 3 considers the experimental systems for studying the expression of isolated eukaryotic genes, concentrating on microinjection into Xenopus oocytes and incubation in cell-free systems in vitro. This book is of great value to genetic engineers, geneticists, and biochemists.
